Composite

Part:BBa_K1157021:Design

Designed by: Lin Ang Chieh   Group: iGEM13_NTU-Taida   (2013-09-16)


pRhl-RhlR-GFP-Pc-RhlR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1712
    Illegal NheI site found at 1735
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 322
    Illegal BamHI site found at 2009
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 797
    Illegal BsaI site found at 2484
    Illegal BsaI.rc site found at 1484


Design Notes

There are detailed design of the sequence, and data has shown that with the reporter placed BEFORE the constitutive promoter, better functional results can be yield.


Source

It is built by the original biobricks produced by iGEM oficial.

References